Heredity and you will Unit Mapping away from Strict-placenta Gene and you will Seed Faculties from inside the Chinese Hami Melon ‘Queen’

Hami melon ‘Queen’ (Cucumis melo ssp. melo var. ameri Pangalo) is the most widely cultivated and exported type of melon in Xinjiang Province, Northwest China. We previously found the unique traits of Hami melon ‘Queen’ for wave seeds and tight-placenta fruits. An analysis of the inheritance showed that these traits were controlled by two recessive genes wave seed (ws) gene and tight-placenta (tp) gene, respectively. Here, to identify these two traits and melon seed–related traits, segregation populations including BCstep step step one and Fdos derived from a cross between ‘Queen’ (P1) and MR-1 (P2) were used as mapping populations. Eighty-seven simple sequence repeat (SSR) markers were used in map construction of BC1P1 population, and as a result, ws and tp were identified on linkage group 1. Analysis of quantitative trait locus (QTL) referring seed traits showed that QTL ss1.1 for seed shape (SS) and QTL sl1.1 for seed length (SL) were located at LG 1, supported by likelihood of odds (LODs) of 15.6 and 13.4, respectively, and both linked with ws. Subsequently, the genetic linkage and parental re-sequence analysis were constructed for fine mapping ws and tp. Genetic analysis showed that ws and tp were located in CM3.5_scaffold00060 on LG 1, flanked by InDelchr1-3241 and InDelchr1-3233. The 80.9-kb physical distance of this region included 11 candidate genes. Among them, MELO3C023549 and MELO3C023551 could be candidates for ws and tp by sequence alignment and allelic variation survey in parental lines. MELO3C023549 was predicted to encode an MYB46-like transcription factor related to positive regulation of secondary cell wall biogenesis. MELO3C023551 was annotated to encode a cellulose synthase A (CESA) associated with cellulose biosynthetic process.

Conceptual

Hami melon ‘Queen’ (Cucumis melo ssp. melo var. ameri Pangalo) is the most widely cultivated and exported type of melon in Xinjiang Province, Northwest China. We previously found the unique traits of Hami melon ‘Queen’ for wave seeds and tight-placenta fruits. An analysis of the inheritance showed that these traits were controlled by two recessive genes wave seed (ws) gene and tight-placenta (tp) gene, respectively. Here, to identify these two traits and melon seed–related traits, segregation populations including BC1 and F2 derived from a cross between ‘Queen’ (P1) and MR-1 (P2) were used as mapping populations. Eighty-seven simple sequence repeat (SSR) markers were http://datingranking.net/it/sesso-occasionale used in map construction of BC1P1 population, and as a result, ws and tp were identified on linkage group 1. Analysis of quantitative trait locus (QTL) referring seed traits showed that QTL ss1.1 for seed shape (SS) and QTL sl1.1 for seed length (SL) were located at LG 1, supported by likelihood of odds (LODs) of 15.6 and 13.4, respectively, and both linked with ws. Subsequently, the genetic linkage and parental re-sequence analysis were constructed for fine mapping ws and tp. Genetic analysis showed that ws and tp were located in CM3.5_scaffold00060 on LG 1, flanked by InDelchr1-3241 and InDelchr1-3233. The 80.9-kb physical distance of this region included 11 candidate genes. Among them, MELO3C023549 and MELO3C023551 could be candidates for ws and tp by sequence alignment and allelic variation survey in parental lines. MELO3C023549 was predicted to encode an MYB46-like transcription factor related to positive regulation of secondary cell wall biogenesis. MELO3C023551 was annotated to encode a cellulose synthase A (CESA) associated with cellulose biosynthetic process.

Heredity and you may Molecular Mapping from Rigid-placenta Gene and Seeds Traits from inside the Chinese Hami Melon ‘Queen’

Melon (C. melo L.; 2n = 2x = 24), a major monetary good fresh fruit harvest, is developed commonly inside the exotic and you can subtropical places. Hami melon ‘Queen’ (C. melo ssp. melo var. ameri Pangalo) is considered the most generally cultivated and you will exported types of melon when you look at the Xinjiang State, Northwest China. Producing nice Hami melon advantages of the fresh climate around, including deceased weather, higher fluctuation regarding day-after-day heat, good sun, and long sunlight period during the summer. Xinjiang Hami melon has actually higher reputation global for the unique flavor and you may maximum sugar content (Ning et al., 2014b).